Journal: International Journal of Oncology

Article Title: SERP1 is a novel marker of poor prognosis in pancreatic ductal adenocarcinoma patients via anti-apoptosis and regulating SRPRB/NF-κB axis

doi: 10.3892/ijo.2017.4111

Figure Lengend Snippet: Flow chart of the derivation and function analysis of stress associated endoplasmic reticulum protein 1 (SERP1). (A) Flow chart. (B) Heat map of differentially expressed mRNAs in pancreatic ductal adenocarcinoma (PDAC) from GEO profiles database. The 10 mRNAs with the most significant difference are shown.

Article Snippet: Human PDAC cell line PANC-1 was purchased from the Cell Resource Center of Beijing Xiehe (Beijing, China) and cultivated in an incubator at 37°C with 5% CO 2 .

Techniques:

Journal: International Journal of Oncology

Article Title: SERP1 is a novel marker of poor prognosis in pancreatic ductal adenocarcinoma patients via anti-apoptosis and regulating SRPRB/NF-κB axis

doi: 10.3892/ijo.2017.4111

Figure Lengend Snippet: Expression level of the stress associated endoplasmic reticulum protein 1 (SERP1) in pancreatic ductal adenocarcinoma (PDAC) tissues is upregulated. (A) SERP1 was upregulated in six pairs of PDAC tissues compared with adjacent normal tissues. (B) The expression of SERP1 in eight pairs of PDAC tissues was upregulated compared with adjacent normal tissues from GEO database. (C and D) The Human Protein Atlas database results revealed that the expression level of SERP1 were upregulated in PDAC tissues compared with normal tissues. (E) Amplification of SERP1 was found in various tumors, including PDAC.

Article Snippet: Human PDAC cell line PANC-1 was purchased from the Cell Resource Center of Beijing Xiehe (Beijing, China) and cultivated in an incubator at 37°C with 5% CO 2 .

Techniques: Expressing, Amplification

Journal: International Journal of Oncology

Article Title: SERP1 is a novel marker of poor prognosis in pancreatic ductal adenocarcinoma patients via anti-apoptosis and regulating SRPRB/NF-κB axis

doi: 10.3892/ijo.2017.4111

Figure Lengend Snippet: Correlation between expression level of the stress associated endoplasmic reticulum protein 1 (SERP1) and progression of pancreatic ductal adenocarcinoma (PDAC) patients. (A and B) The expression level of SERP1 in T3/4 stage PDAC specimen was significantly higher than in T1/2 stage, while there was no significant difference between SERP1 and N stage. (C) Correlation analysis was performed between expression level of SERP1 and clinical stages. SERP1 was correspondingly increased with the increase of clinical AJCC stage of PDAC. (D) Constituent ratio with high level of SERP1 was drastically different between normal samples and I/II or III/IV stage of PDAC patients.

Article Snippet: Human PDAC cell line PANC-1 was purchased from the Cell Resource Center of Beijing Xiehe (Beijing, China) and cultivated in an incubator at 37°C with 5% CO 2 .

Techniques: Expressing

Journal: International Journal of Oncology

Article Title: SERP1 is a novel marker of poor prognosis in pancreatic ductal adenocarcinoma patients via anti-apoptosis and regulating SRPRB/NF-κB axis

doi: 10.3892/ijo.2017.4111

Figure Lengend Snippet: The correlation between expression level of the stress associated endoplasmic reticulum protein 1 (SERP1) and prognosis of pancreatic ductal adenocarcinoma (PDAC) patients. (A) K-M curve for OS of patients with high (n=89) and low (n=87) SERP1 expression level. Results revealed that high SERP1 expression level was correlated with shorter OS for PDAC patients. (B) SERP1 high expression group (n=61) had shorter DFS than SERP1 low expression group (n=76). (C) The expression of SERP1 in OS-good and poor patients. (D) The expression of SERP1 in DFS-good and poor patients. (E and F) Multivariate Cox regression analyses showed SERP1 expression was an independent factor of patients' overall survival and disease-free survival.

Article Snippet: Human PDAC cell line PANC-1 was purchased from the Cell Resource Center of Beijing Xiehe (Beijing, China) and cultivated in an incubator at 37°C with 5% CO 2 .

Techniques: Expressing

Journal: International Journal of Oncology

Article Title: SERP1 is a novel marker of poor prognosis in pancreatic ductal adenocarcinoma patients via anti-apoptosis and regulating SRPRB/NF-κB axis

doi: 10.3892/ijo.2017.4111

Figure Lengend Snippet: Downregulated stress associated endoplasmic reticulum protein 1 (SERP1) promotes cell apoptosis via regulating SRP receptor β subunit (SRPRB) associated NF-κB activation. (A) NF-κB signaling pathway gene sets were enriched in the tumor group. (B) Correlation analysis revealed that the expression level of SERP1 positively correlated with NF-κB expression in 178 pancreatic ductal adenocarcinoma (PDAC) patients from TCGA database. (C) The expression level of SRPRB was negatively correlated with NF-κB expression in 178 PDAC patients from TCGA database. (D) Western blotting confirmed that downregulated SERP1 could suppress the expression of NF-κB and the phosphorylation NF-κB in PANC-1 cells.

Article Snippet: Human PDAC cell line PANC-1 was purchased from the Cell Resource Center of Beijing Xiehe (Beijing, China) and cultivated in an incubator at 37°C with 5% CO 2 .

Techniques: Activation Assay, Expressing, Western Blot, Phospho-proteomics

Journal: Signal Transduction and Targeted Therapy

Article Title: Neoleukin-2/15-armored CAR-NK cells sustain superior therapeutic efficacy in solid tumors via c-Myc/NRF1 activation

doi: 10.1038/s41392-025-02158-2

Figure Lengend Snippet: Neo-2/15-armored CAR-NK cells exhibit potent antitumor efficacy in vitro and in vivo. a Schematic representation of the lentivirus vectors encoding the αMSLN-CAR (upper) and Neo-2/15 (lower). Transmembrane region: CD8, co-stimulating domain: 4-1BB and CD3ζ, promoter: CMV or EF1α, SP: signal peptide. b Cytotoxicity of NK-92, BBζ and BBζ-Neo toward AsPC-1, MSLN OE PANC1 or MSLN OE BxPC-3 cells after co-culture for 4 hours at E:T ratios of 1:1 or 5:1 detected by the LDH assay. Data are presented as mean ± SD (* P < 0.05, ** P < 0.01, *** P < 0.001 comparing BBζ-Neo with NK-92, # P < 0.05, ## P < 0.01, comparing BBζ-Neo with BBζ, n = 4). Quantification of the expression of PFN and GrB ( c ) and CD107a ( d ) in NK-92, BBζ or BBζ-Neo after co-cultured with AsPC-1 cells for 4 hours. Data are presented as mean ± SD (* P < 0.05, ** P < 0.01, n = 3). e IFNγ secreted by NK-92, BBζ or BBζ-Neo after co-cultured with AsPC-1 cells for 4 hours at the E:T ratio of 1:1 detected by ELISA. Data are presented as mean ± SD (* P < 0.05, ** P < 0.01, *** P < 0.001, n = 3). f Cytokine array analysis of cytokines secreted by BBζ or BBζ-Neo after co-cultured with or without AsPC-1 cells for 4 hours. g Representative images of the morphology of PDO after 8-hour co-culture with BBζ, or BBζ-Neo at the E:T ratio of 4:1. Red arrows indicate PDO that are efficiently lysed. Insets show enlarged images (left). Lysis efficiency was assessed by enumerating the lysed cells within each field of view (right). Lysis (%) = (lysed cells / total cells) × 100%. Data are presented as mean ± SD (* P < 0.05, n = 3). h Schematic representation of the evaluation of antitumor activity of NK-92, BBζ or BBζ-Neo in vivo. NCG mice were engrafted with 5 × 10 6 AsPC-1 cells on day 0. The tumor-bearing mice received NK-92, BBζ or BBζ-Neo (1 × 10 7 per mouse) on day-7, -14 and -21. On the day-12 after tumor inoculation, some mice were euthanized and intratumoral NK cells were detected by multicolor immunofluorescence (mIF). Tumor growth ( i ) and survival ( j ) of tumor-bearing mice treated with PBS (control), NK-92, BBζ or BBζ-Neo. Data are presented as mean ± SD (ns, not significant, * P < 0.05, ** P < 0.01, *** P < 0.001 compared with NK-92, ### P < 0.001 compared with BBζ, n = 6). k Schematic outline of the development of the modified orthotopic PDAC model and evaluation of the antitumor activity of various NK cells. The tumor-bearing mice received NK-92, BBζ or BBζ-Neo (1 × 10 7 per mouse) on day-7, -14 and -21 (created using BioRender). l The tumor burden in tumor-bearing mice were monitored by measuring luminescence using IVIS. m Overall kinetics of systemic tumor progression in mice are plotted. Data are presented as mean ± SD (ns, not significant, * P < 0.05, n = 6). n Kaplan-Meier curve representing the percent survival of the indicated experimental groups. Statistics: two-tailed log rank test. Data are presented as mean ± SD (* P < 0.05, ** P < 0.01, n = 6)

Article Snippet: MSLN overexpressing cell lines (MSLN OE PANC-1 and MSLN OE BxPC-3) were generated by lentiviral transduction (Genepharma, LV2021-11386).

Techniques: In Vitro, In Vivo, Co-Culture Assay, Lactate Dehydrogenase Assay, Expressing, Cell Culture, Enzyme-linked Immunosorbent Assay, Lysis, Activity Assay, Immunofluorescence, Control, Modification, Two Tailed Test

Journal: Signal Transduction and Targeted Therapy

Article Title: Neoleukin-2/15-armored CAR-NK cells sustain superior therapeutic efficacy in solid tumors via c-Myc/NRF1 activation

doi: 10.1038/s41392-025-02158-2

Figure Lengend Snippet: Neo-2/15 augments the expression of nutrient transporters and OXPHOS of CAR-NK cells. a , b Immunoblot for JAKs/STATs and Akt/mTOR pathways in BBζ stimulated with IL-2 or Neo-2/15 in the absence ( a ) or presence ( b ) of AsPC-1 cells. c The mRNA (top) and protein (bottom) levels of c-Myc in Neo-2/15 stimulated BBζ pretreated with Stafia1 (22 μM), AZD5363 (8 nM) or Rapamycin (10 nM) alone or in combination in the same concentration overnight were evaluated. Western blots are representative of three independent experiments. Data are presented as mean ± SD (ns, not significant, * P < 0.05, ** P < 0.01, *** P < 0.001 compared with DMSO group, ## P < 0.01, ### P < 0.001 compared with Stafia1 group, n = 3). d Heatmap showing the cytolytic activity against MSLN positive tumor cells of Neo-2/15 stimulated BBζ pretreated with Fludarabine (50 μM), NSC74859 (86 μM), Stafia1 (22 μM), AZD5363 (8 nM), Rapamycin (10 nM), 10058-F4 (60 μM), Nifuroxazide (3 μM), Stafia1 (22 μM) + AZD5363 (8 nM), or Rapamycin (10 nM) + Stafia1 (22 μM) overnight measured by the LDH assay. e ATP generation in BBζ stimulated with IL-2 or Neo-2/15 without (left) or with (middle) co-cultured with AsPC-1 cells for 4 hours or in Neo-2/15 stimulated BBζ pretreated with 10058-F4 (60 μM) for 30 minutes and then co-cultured with AsPC-1 cells for 4 hours (right). Data are presented as mean ± SD (*** P < 0.001, n = 5). f Representation of pathways altered in IL-2 or Neo-2/15 stimulated BBζ based on transcriptome data. Pathways of interest are highlighted. g OCR was assessed using a seahorse analyzer in BBζ stimulated with either Neo-2/15 or IL-2 after co-culturing with AsPC-1 cells. h OCR was assessed in Neo-2/15 expanded BBζ pre-treated with or without 10058-F4 (60 μM) and subsequently co-cultured with AsPC-1 cells. i mRNA (top) and protein (bottom) levels of the nutrient transporters in Neo-2/15 or IL-2 stimulated BBζ without or with co-cultured with AsPC-1 cells. Blots are representative of three independent experiments. Data are presented as mean ± SD (** P < 0.01, *** P < 0.001, n = 5). j mRNA (top) and protein (bottom) levels of the nutrient transporters in Neo-2/15 stimulated BBζ pretreated with Stafia1 (22 μM), AZD5363 (8 nM) or 10058-F4 (60 μM) for 30 minutes after co-cultured with AsPC-1 cells. Blots are representative of three independent experiments. Data are presented as mean ± SD (ns, not significant, * P < 0.05, *** P < 0.001 compared with DMSO group, ## P < 0.01, ### P < 0.001 compared with Stafia1 group, n = 5)

Article Snippet: MSLN overexpressing cell lines (MSLN OE PANC-1 and MSLN OE BxPC-3) were generated by lentiviral transduction (Genepharma, LV2021-11386).

Techniques: Expressing, Western Blot, Concentration Assay, Activity Assay, Lactate Dehydrogenase Assay, Cell Culture

Journal: Cancer Immunology, Immunotherapy : CII

Article Title: Allogeneic tumor lysate can serve as both antigen source and protein supplementation for dendritic cell culture

doi: 10.1007/s00262-007-0422-0

Figure Lengend Snippet: Intracellular IFN-γ-production and cytotoxic activity of T-cells stimulated with FBS-DC or TuLy-DC. a Freshly isolated PBMC from MTC patients undergoing immunotherapy were co-cultured either with mature autologous FBS-DC or TuLy-DC. After 24 h of incubation, PBMC were stained and analyzed by flow cytometry. Percentages of CD3+-, CD4+- and CD8+T-cells positive for IFN-γ are indicated in the plots. b Freshly isolated CD3+T-cells cells were co-cultured either with (filled rectangle) mature autologous tumor lysate-pulsed FBS-DC or (open circle)TuLy-DC. CD3+T cells cultured without DC (filled triangle) or unloaded FBS-DC (open triangle) served as controls. After 6 days of incubation, cytotoxic activity of T cells against SHER-I, PANC-1 (pancreatic cancer cell line) and K-562 (chronic myelogenous leukemia cells) was evaluated using a 4-h europium release assay

Article Snippet: Before preparation of lysate SHER-I cells were extensively washed and cultured in the same medium as before but without supplementation of FCS for at least 48 h. PANC-1 and K-562 cell lines (LGC Promochem—ATCC, Teddington, UK) were cultured in RPMI 1640 medium + l -glutamine (Gibco) supplemented with 10% FBS and 50 μg/ml gentamycin in a 5% CO 2 humidified atmosphere at 37°C.

Techniques: Activity Assay, Isolation, Cell Culture, Incubation, Staining, Flow Cytometry, Release Assay